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Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (3): 380-384.

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Intestinal Transport and Biotransformation of Resibufogenin and Cinobufagin in Chan Su via HPLC/APCI-MSn

HAN Tian-jiao1,4, WANG Qing2, SONG Feng-rui1, LIU Zhong-ying3, LIU Zhi-qiang1* and LIU Shu-ying1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, P. R. China;
    2. Department of Endocrinology of China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    3. School of Pharmacy, Jilin University, Changchun 130033, P. R. China;
    4. Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China
  • Received:2010-09-06 Revised:2010-12-10 Online:2011-05-25 Published:2011-04-29
  • Contact: LIU Zhi-qiang E-mail:liuzq@ciac.jl.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30772721 and 30873360).

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Abstract: In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.

Key words: Chan Su, Resibufogenin, Cinobufagin, Caco-2 cell monolayer, Human intestinal bacteria