CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (2): 193-197.

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Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell

WANG Jin-e1,2, WANG Cheng-ke1,2, LIU Dian-jun1 and WANG Zhen-xin1*   

  1. 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    2. Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
  • Received:2010-07-22 Revised:2010-09-16 Online:2011-03-25 Published:2011-03-09
  • Contact: WANG Zhen-xin E-mail:wangzx@ciac.jl.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20875087) and the Fund of Chinese Academy of Sciences (No.KJCX2-YW-H11).

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Abstract: The interactions of antibiotic with living cells were studied by lectin conjugated gold nanoparticles(GNPs) based colorimetric assay. Because of the high affinity of lectin for saccharides, the lectin conjugated GNPs are able to employ as indicators for monitoring the antibiotic induced changes of glycosyl complexes. The interactions of a well known antibiotic, tunicamycin, with two different cell lines, HeLa and SHG-44, were selected to establish this assay. In the presence of tunicamycin, the dose- and time-dependence on the decreasing of binding affinity of lectin conjugated GNPs with living cells were demonstrated by conventional microscopic and UV-Vis spectroscopic studies. The experimental result demonstrates that our approach can be used to identify antibiotic induced expression difference of glycosyl complexes on different cellular surfaces and determine drug activity quantitatively. For further confirming the capability of the GNP-based assay, the system was also studied by confocal laser scanning microscopy(CLSM) and classic flow cytometry(FCM) assay, and satisfactory results were obtained.

Key words: Lectin-conjugated gold nanoparticle, Tunicamycin, Colorimetric assay, Living cell