A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells

doi:10.1007/s40242-019-9246-7

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (6): 967-971.doi: 10.1007/s40242-019-9246-7

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A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells

WANG Shuxin¹, CAO Jian¹, CHENG Yuxiao², LU Chenhong¹

1. School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, P. R. China;
2. Technical Center for Industrial Product and Raw Material Inspection and Testing, Shanghai Customs, Shanghai 200135, P. R. China

Received:2019-09-11 Revised:2019-10-20 Online:2019-12-01 Published:2019-11-29
Contact: CAO Jian, LU Chenhong E-mail:caoj@sues.edu.cn;luchenhong25@foxmail.com
Supported by:
Supported by the National Key Research and Development Program of China(No.2016YFF0203704), the Innovation Fund for Graduate Students of Shanghai University of Engineering Science, China(Nos.18KY0416, 16KY0410) and the National Natural Science Foundation of China(No.21404068).

Abstract

Abstract: A highly sensitive fluorescent "turn-on" probe NapP-deap based on naphthalimide derivative was deve-loped that bound Hg²⁺ ions rapidly in the N-2-hydroxyethylpiperazine-N-ethane-sulphonic acid(HEPES) buffer solution via photo-induced electron transfer(PET) being inhibited mechanism. The titration experiment displayed that the emission intensity of NapP-deap at 540 nm was almost linearly increased by about 3-fold. The Job's plot showed a stoichiometry factor of 1:1 of the ligand-to-metal ratio. The detection limit of fluorescent probe was calculated to be 6.2×10^-9 mol/L. ¹H NMR studies could confirm that one Hg²⁺ ion was bound by the N atoms(a, b) of piperazine or the N atom(c) of pyridine. The fluorescent probe could be used for the detection of Hg²⁺ ions in living cells.

Key words: Fluorescent “turn-on”, Hg²⁺, HEPES buffer solution, Living cell, PET mechanism

WANG Shuxin, CAO Jian, CHENG Yuxiao, LU Chenhong. A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells[J]. Chemical Research in Chinese Universities, 2019, 35(6): 967-971.

References

[1] Ueno A., Instrumentation Science & Technology, 1993, 22(4), 405
[2] Huang C. B., Fan J. L., Peng X. J., Lin Z. Y., Guo B. P., Ren A. X., Journal of Photochemistry & Photobiology A Chemistry, 2008, 199(2), 144
[3] Brümmer O., La Clair J. J., Janda K. D., Bioorganic & Medicinal Chemistry, 2001, 9(4), 1067
[4] Kim S. K., Swamy K. M. K., Chung S. Y., Kim H. N., Min J. K., Jeong Y. S., Tetrahedron Letters, 2010, 51(25), 3286
[5] Boening D. W., Chemosphere, 2000, 40(12), 1335
[6] Nolan E. M., Lippard S. J., Chem.Rev., 2008, 108(9), 3443
[7] Pandey S. K., Kim K. H., Brown R. J. C., Trac. Trends in Analytical Chemistry, 2011, 30(6), 899
[8] dos Santos J. S., De L. G. M., Pastor A., dos Santos M. L., Talanta, 2010, 80(1), 207
[9] Ros-Lis J. V., Marcos M. D., Mártinez-Máñez R., Rurack K., Soto J., Angew. Chem. In. Ed., 2010, 44(28), 4405
[10] Coskun A., Akkaya E. U., Journal American Chemical Society, 2006, 128(45), 14474
[11] Zhang X., Xiao Y., Qian X., Angew. Chem. In. Ed., 2008, 47(42), 8025
[12] Neupane L. N., Kim J. M., Lohani C. R., Lee K. H., J. Mater. Chem., 2012, 22(9), 4003
[13] Ono A., Togashi H., Angew. Chem. In. Ed., 2010, 43(33), 4300
[14] Ko S. K., Yang Y. K., Tae J., Shin I., J. Am. Chem. Soc., 2006, 128(43), 14150
[15] Min H. L., Lee S. W., Sang H. K., Kang C. H., Kim J. S., Organic Letter, 2009, 11(10), 2101
[16] Wang H., Li Y., Xu S., Li Y., Zhou C., Fei X., Organic Biomolecular Chemistry, 2011, 9(8), 2850
[17] Lin W., Cao X., Ding Y., Yuan L., Long L., Chem. Commun., 2010, 46(20), 3529
[18] Kim H. N., Ren W. X., Kim J. S., Yoon J., Chem. Soc. Rev., 2012, 41(8), 3210
[19] Wang S. S., Lin B., Chen L., Li N., Xu J. J., Wang J., Anal. Chem., 2018, 90(20), 11764
[20] Malek A., Bera K., Biswas S., Perumal G., Das A. K., Doble M., Anal. Chem., 2019, 91(5), 3533
[21] Wang Y., Pan F., Zhang Y., Peng F., Huang Z., Zhang W., Analyst, 2016, 141(15), 4789
[22] Lee J. J., Kim Y. S., Nam E., Lee S. Y., Lim M. H., Kim C., Dalton Transactions, 2016, 45(13), 5700
[23] Lei L., Tan X., Luo S. L., Shi C. M., New Journal of Chemistry, 2017, 41(17), 8899
[24] Zhang G., Zhang D., Yin S., Yang X., Shuai Z., Zhu D., Chem. Commun., 2005, 16(16), 2161
[25] Kim J. S., Choi M. G., Song K. C., No K. T., Ahn S., Chang S. K., Organic Letters, 2007, 9(6), 1129
[26] Ha-Thi M. H., Penhoat M., Michelet V., Leray I., Organic Letters, 2007, 9(6), 1133
[27] Song F. L., Watanabe S. J., Floreancig P. E., Koide K., J. Am. Chem. Soc., 2008, 130(49), 16460
[28] Zhou Y., Zhu C. Y., Gao X. S., You X. Y., Yao C., Organic Letters, 2010, 12(11), 2566
[29] Bag B., Pal A., Organic Biomolecular Chemistry, 2011, 9(12), 4467
[30] Zhang S. F., Geng J. M., Yang W., Zhang X. L., RSC Advance, 2014, 4(24), 12596
[31] Tsukamoto K., Shinohara Y., Iwasaki S., Maeda H., Chem. Commun., 2011, 47(17), 5073
[32] Lee J. W., Jung H. S., Kwon P. S., Kim J. W., Bartsch R. A., Kim Y., Organic Letters, 2008, 10(17), 3801
[33] Du J. J., Fan J. L., Peng X. J., Sun P. P., Wang J. Y., Li H. L., Organic Letters, 2010, 12(3), 476
[34] Liu F., Tang P., Ding R., Liao L., Wang L., Wang M., Dalton Transactions, 2017, 46(23), 7515
[35] Wang J. B., Xiao Y., Zhang Z. C., Qian X. H., Yang Y. Y., Xu Q., J. Mater. Chem., 2005, 15(27/28), 2836

A Sensitive Fluorescent Turn-on Probe NapP-deap Based on Naphthalimide Derivative to Detect Hg(II) Ions in HEPES Buffer Solution and Living Cells

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