CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (1): 99-103.

• Articles • Previous Articles     Next Articles

Aquaporin 1 Facilitated Hepatocellular Carcinoma SMMC7221 Cell Migration Associated with Water Permeability

ZHANG Ai-li1, LI Jiang2, WANG Yan-qing1, ZAKNROU Zohra1, MA Tong-hui1,4 and LI Xiao-meng1,3*   

  1. 1. School of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China;
    2. Dental Hospital, Jilin University, Changchun 130021, P. R. China;
    3. Bo’an Brothers Holding Pharmaceutical Co., Ltd.(China), Changchun 130012, P. R. China;
    4. Central Research Laboratory, The Second Hospital of Jilin University, Changchun 130041, P. R. China
  • Received:2010-01-13 Revised:2010-08-17 Online:2011-01-25 Published:2011-01-04
  • Contact: LI Xiao-meng E-mail:lixm441@nenu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos. 30871301，30700827), the Program of Ministry of Science and Technology of China(No.2010DFA31430), the Program of Jilin Provincial Science & Technology Department, China(Nos.20070719, 20080731 and 200905116) and the Fund of Northeast Normal University, China(No.NENU-STC07005).

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Abstract: The authors investigated the regulation of human aquaporin 1(hAQP1) and the involvement of aquaporin 1(AQP1) in the migration of human hepatocellular carcinoma SMMC-7221 cells using RNA intereference technology. Firstly, two short hairpin RNA(shRNA) constructs in PBSU6 vector were reconstructed and their knockdown effects were identified in SMMC-7221 cells. Next, the involvement of endogenous hAQP1 in regulating the migration of SMMC-7221 cells was investigated via siRNA technology. HAQP1-shRNA can specifically inhibit AQP1 dependent osmotic water permeability. Meanwhile the migration of SMMC-7221 cells was inhibited remarkably after silencing AQP1 by performing transwell cell migration assay and in vitro wound healing assay. Furthermore, in the presence of an inhibitor HgCl2, the water permeability of the cell membrane was remarkably decreased, the expression of AQP1 was upregulated after HgCl2 treatment and the cell movement was decreased at the moment. Increased AQP1 cannot attenuate cell migration ability when cell membrane loses its water permeability function. This demonstrates that the cell migration was remarkably related to the transporting water function of cell membrane.

Key words: Aquaporin 1, shRNA, HgCl2, Cell migration