CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (1): 104-107.

• Articles • Previous Articles     Next Articles

Structural Analysis of an Oligosaccharide and Glycopeptide Mixture from Panax Ginseng Root with Inhibition SHP-1 Function

WANG Ying1, GAO Qi-pin2, LI Gui-rong3, CHEN Ying-hong4, LUO Hao-ming4, GAO Yang2 and JIANG Rui-zhi4*   

  1. 1. College of Light Industry and Food, South China University of Technology, Guangzhou 510641, P. R. China;
    2. Center for New Medicine Research, Changchun University of Chinese Medicine, Changchun 130117, P. R. China;
    3. School of Pharmacy, Yanbian University, Yanji 133000, P. R. China;
    4. Academy of Chinese Medicine and Material Medica of Jilin Province, Changchun 130021, P. R. China
  • Received:2010-01-06 Revised:2010-05-10 Online:2011-01-25 Published:2011-01-04
  • Contact: JIANG Rui-zhi E-mail:zhongyanjrz@yahoo.com.cn
  • Supported by:

    Supported by the National Science & Technology Pillar Program of China(No.2007BA138B00).

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Abstract: A mixture of oligosaccharide and glycopeptide was isolated from the aqueous extract of Panax ginseng roots. The mixture inhibits protein tyrosine phosphatase(SHP-1) function, implying it enhances immune activity. The peak molecular mass of the oligosaccharide portion is 1800 calculated via GPC software after separation by HPLC. And the structure of the oligosaccharide portion is the backbone of (1→3)- and (1→4)-linked arabinopyranoside, and (1→4)- and (1→6)-linked glucopyranoside, with non-reducing terminals of arabinopyranoside and glucopyranoside. The peak molecular mass of glycopeptide portion is 1900 calculated via GPC software after separation by HPLC. The structure of glycopeptide portion is the backbone of (1→3)- and (1→4)-linked arabinopyranoside, and (1→3,6)-linked glucopyranoside, with non-reducing terminals of galactopyranose and glucopyranoside. The peptide composition is Glu, Asp, Hyp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Ile, Leu and Lys. The oligosaccharide-peptide linkage is formed by Ara and Hyp.

Key words: Panax ginseng, Oligosaccharide, Glycopeptide, Protein tyrosine phosphatase(SHP-1), Methylation analysis