CRCU

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (2): 252-255.

• Articles • Previous Articles     Next Articles

Proteomics Approach to Investigate Impact of aFGF Antagonist on Signal Transduction Pathway

FU Yu-qin1,3 , LI Min1,2, WANG Xu2, ZHOU Guo-qiang1, ZHOU Hui1 and CUI Yin-qiu1*   

  1. 1. Biomacromolecular Laboratory,
    2. Key Lab of Molecular Enzymology Engineening, Ministry of Education, College of Life Science, Jilin University, Changchun 130021, P. R. China;
    3. College of Life Science, Jilin Agricultural University, Changchun 130118, P. R. China
  • Received:2009-03-30 Revised:2009-04-09 Online:2010-03-25 Published:2010-05-25
  • Contact: CUI Yin-qiu. E-mail: yinqiucui@yahoo.com.cn
  • Supported by:

    Supported by the Science and Technology Development Project of Jilin Province, China(No.20040411-3).

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Cited

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Abstract:

Fibroblast growth factor(FGF) signal transduction pathway is correlated with many diseases, such as cancer, skeletal dysplasias, olfactory syndromes and so on. P(VYMSPF), a small peptide, is an antagonist of aFGF. In order to investigate the impact of the peptide P on aFGF signal transduction pathway, the proteomes of NIH3T3 cell stimulated under different conditions(aFGF+heparin sodium and aFGF+heparin sodium+P) were extracted and analyzed by 2DE, five differentially expressed protein spots were selected and identified via ESI-MS/MS. The result indicates that the peptide P has a great influence on the function of aFGF such as metabolism, protein translation and cytoskeleton formation; the target signal pathway of the peptide P may be MAPK pathway and the peptide P might also affect the PLCγ pathway. The article provides a new insight into the peptide P as a potential anticancer drug.

Key words: Proteomics; aFGF; Signal transduction pathway