Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (2): 230-234.

• Articles • Previous Articles     Next Articles

Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C. A. Meyer

NI Xiu-zhen1,2, WANG Bing-qing1, ZHI Yuan1, WEI Ning-ning1, ZHANG Xu1, LI Shan-shan1, TAI Gui-hua1, ZHOU Yi-fa1* and ZHAO Ji-ming2*   

  1. 1. School of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China;
    2. School of Life Sciences, Changchun Normal University, Changchun 130032, P. R. China
  • Received:2009-04-20 Revised:2009-05-18 Online:2010-03-25 Published:2010-05-25
  • Contact: ZHOU Yi-fa. E-mail: zhouyf383@nenu.edu.cn; ZHAO Ji-ming. E-mail: zmj@263.net
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30570417 and 30973857), the Natural Science Foundation of Jilin Province(No.20070710), the Natural Science Foundation of Changchun, China(Nos.2009081 and 09SF03).

Abstract:

The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction(GLPN-1), six acidic fractions(GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3) by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1:1, 1:2 and 1:2.7, respectively, indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin. GLPA-1c was mainly composed of GalA(78%), belonging to homogalacturonan(HG)-rich pectin. GLPA-3 might be a glycoprotein.

Key words: Ginseng leave; Polysaccharide; Fractionation; RG-I domain