CRCU

Chemical Research in Chinese Universities ›› 2006, Vol. 22 ›› Issue (4): 479-483.

• Articles • Previous Articles     Next Articles

Sensitive and Rapid Quantification of Felodipine by High-performance Liquid Chromatography-tandem Mass Spectrometry(HPLC-MS/MS) and Its Pharmacokinetics in Healthy Chinese Volunteers

LI Yan-yan1,2, YIN Yi-zi1, SUN Zhi-hui1, LI Xin2, HU Li-gang2, LI Peng-fei2, ZHONG Da-fang2   

  1. 1. The First Hospital, Jilin University, Changchun 130021, P.R. China;

    2. Research Center for Drug Metabolism, Jilin University, Changchun 130021, P. R. China
  • Received:2006-01-16 Online:2006-08-24 Published:2011-08-06
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 30472053).

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Abstract: To investigate the pharmacokinetics of felodipine in the plasma of healthy Chinese volunteers, 30 healthy volunteers received a single oral dose of 5 mg of extended release felodipine tablets. The felodipine was extracted from the matrix with a liquid-liquid extract procedure and analyzed by high-performance liquid chromatography-tandem mass spectrometry in the multiple reaction monitoring(MRM) mode using an electrospray ion source with positive ion detection. The method was validated over a felodipine concentration range of 0.05-10.00 ng/mL in human plasma. Its main pharmacokinetic parameters values were: ρmax=(1.67±0.84) ng/mL, occurring at (3.93±2.49) h; the plasma elimination half-life: (23.08±9.48) h and the area under the plasma concentration versus time curve: (29.94±14.39) ng·h/mL. The validation results demonstrated that this method showed a satisfactory precision and accuracy across the calibration range. The procedure involved minimal drug administration, sample preparation, and a 2.5-min chromatographic run time. It was well suited to clinical studies of the drug involving large numbers of samples.

Key words: Felodipine, Liquid chromatography-tandem mass spectrometry, Pharmacokinetics