CRCU

Chemical Research in Chinese Universities ›› 2005, Vol. 21 ›› Issue (4): 436-438.

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Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis

LIU Ning1,2, LÜ Lei1, ZHANG Xuan1, WANG Zhi3, SUN Ya-dong3, LIU Zhi-qiang1, LIU Shu-ying1   

  1. 1. Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    2. Institute of Life Science and Technology, Jilin University, Changchun 130021, P.R. China;
    3. Key Laboratory of Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130021, P. R. China
  • Received:2004-09-29 Online:2005-07-24 Published:2011-08-06
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.20173057 and 20273067).

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Abstract: A liquid-phase isoelectric focusing electrophoresis system(Rotofor) was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture. Each fraction collected was then directly subjected to MALDI-TOF-MS analysis. By this approach, we are able to resolve two types of hemoglobins, A and C, which cannot be successfully separated by means of the traditional SDS-PAGE method.

Key words: Liquid-phase isoelectric focusing electrophoresis, MALDI-MS analysis, Hemoglobin A and C, Cytochrome c