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Chemical Research in Chinese Universities ›› 2004, Vol. 20 ›› Issue (5): 584-587.

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Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties

TENG Li-rong1, LIANG Fang1, SUN Hong2, QIN Yi3, HONG Shui-sheng1, CHEN Jia1, LIU Lan-ying1   

  1. 1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
    2. Institute of Biological Products, Changchun 130062, P. R. China;
    3. Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, P. R. China
  • Received:2003-12-28 Online:2004-10-24 Published:2011-08-06
  • Supported by:

    Supported by the Natural Science Foundation of Jilin Province(No.2003-05-50-2) and the Creative Foundation of Jilin University.

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Abstract: The dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg).Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier.The new method significantly increases the product yield and purity over the liquidphase methods.The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits.

Key words: Acyl-plasminogen-streptokinase activator complex (APSAC), Solid-phase synthesis, Thrombolytic