Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures

Chemical Research in Chinese Universities ›› 2002, Vol. 18 ›› Issue (3): 294-298.

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Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures

GOU Xiao-jun¹, LI Chun-lei¹, KONG Xiang-duo¹, ZHU Shi-zhen¹, ZHANG Hong-ying¹, WANG Xiao-ping¹, ZHANG Jin¹, LIU Tian-yun²

1. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, P. R. China;
2. The People's Hospital of Tongjiang Country, Tongjiang 635700, P. R. China

Received:2001-07-25 Online:2002-08-24 Published:2011-08-04

Abstract

Abstract: Cu,Zn-SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn-SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn-SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.

Key words: Human Cu,Zn-SOD, Directed evolution, Screening

GOU Xiao-jun, LI Chun-lei, KONG Xiang-duo, ZHU Shi-zhen, ZHANG Hong-ying, WANG Xiao-ping, ZHANG Jin, LIU Tian-yun. Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures[J]. Chemical Research in Chinese Universities, 2002, 18(3): 294-298.

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Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures

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