CRCU

Chemical Research in Chinese Universities ›› 2002, Vol. 18 ›› Issue (3): 290-293.

• Articles • Previous Articles     Next Articles

Cloning of Promoter of Chinese Bean GRP 1.8 Gene and Characterization of Its Function in Transgenic Tobacco Plants

LU Hai1, JIANG Xiang-ning1, LI Feng-lan1, ZENG Qing-yin2, LIU Wei2, GOU Xiao-jun2   

  1. 1. Experimental Center of Forest Biology, College of Plant Sciences, Beijing Forestry University, Beijing 100083, P. R. China;
    2. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, P. R. China
  • Received:2001-06-16 Online:2002-08-24 Published:2011-08-04
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.39730350).

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Abstract: In order to learn the expression pattern of GRP1.8(glycine-rich-protein) gene promoter in transgenic plants and to explore its potential application in plant genetic engineering for vascular-specific expression of interested genes, GRP 1.8 promoter was amplified by PCR from Chinese bean genomic DNA. The intermediate vector was constructed by inserting vascular-specific expression promoter of GRP 1.8 gene in vector pBI 101. The regenerated tobacco plants obtained were analyzed by PCR to select the putative transgenic plants. The histochemical localization of GUS(β-D-glucosidase) activity indicates that as for that of GRP 1.8 promoter we can confer the vascular-specific expression of GUS gene.

Key words: Chinese Bean, Promoter of GRP 1.8 gene, Transgenic tobacco plants