A High Selective Chemiluminescence Reacton of Guanine and Its Analytical Application

Chemical Research in Chinese Universities ›› 1996, Vol. 12 ›› Issue (3): 253-257.

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A High Selective Chemiluminescence Reacton of Guanine and Its Analytical Application

CI Yun-xiang, LI Jia, ZHEN Yuan-gang, LI Yuan-zong

Department of Chemistry, Peking University, Beijing, 100871

Received:1995-10-24 Online:1996-07-15 Published:2011-11-18
Supported by:
Supported by the National Natural Science Foundation of China.

Abstract

Abstract: A high selective quantitative method for the determination of guanine was established based on a well-known copper-phenanthroline complex mediated oxidation of DNA.The affecting factors for the reaction were studied in a HAc-NaAc buffer solution at 37℃. The optimized conditions for the quantitative determination of guanine are: pH5.2; [Cu²⁺]=80μmol/L; [ascorbic acid]=0.4μmol/L; [phen]=7μmol/L and 1.1%H₂O₂.Guanine could be determined in the range of 1.0-10.0μmol/L.The recoveries of guanine in synthetic sample were within 90%-110% with up to 6 times' excess of other bases.This method is simple,fast and selective.It may be used as a routine method for the determination of guanine without the perseparation of other nucleic acid bases.

Key words: Chemiluminescence, Guanine, Copper-phenanthroline complex

CI Yun-xiang, LI Jia, ZHEN Yuan-gang, LI Yuan-zong. A High Selective Chemiluminescence Reacton of Guanine and Its Analytical Application[J]. Chemical Research in Chinese Universities, 1996, 12(3): 253-257.

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A High Selective Chemiluminescence Reacton of Guanine and Its Analytical Application

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