CRCU

Chemical Research in Chinese Universities ›› 1995, Vol. 11 ›› Issue (3): 215-218.

• Articles • Previous Articles     Next Articles

Studies on HEPES Stabilizing M Intermediate of Bacteriorhodopsin

FU Xue-qi1, LI Zheng-qiang2, WANG Jin-dui1, WANG BaO-huia3, LI Bo-fu2, LI Tie-jin3   

  1. 1. National Key Laboratory of Enzyme Engineering;
    2. Key Laboratory of Molecular Spectrum and Molecular Structure;
    3. Department of Chemistry, Jilin University, Changchun, 130023
  • Received:1994-08-30 Online:1995-08-24 Published:2011-08-17

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Abstract: HEPES has proved to stabilize Mintermediate of Bacteriorhodopsin.Absorp-tion and Raman spectra showed that there was a more stable form of Mintermedi-ate in BR-HEPES solution.It was found by SPSthat there were two main interme-diates .M and O, existing in BR-HEPES solution.

Key words: Bacteriorhodopsin, Mintermediate, HEPES