Studies on Artificial Imitation of Glutathione Peroxidase by Chemical Mutation

Abstract

Abstract: After the alcoholic group of serine located in the binding site of Anti-HumanIgM(Fcμ) (Rabbit IgG) was selectively activated by phenylmethylsulfonyl fluoride(PMSF) and displaced with hydrogen selenide(H₂Se), the serine residue was con-verted to selenocysteine(SeCys).Because SeCys is a catalytic group of glutathioneperoxidase (GPX), the mutated antibody has the GPXactivity which is seventytimes more than that of PZ51, the best GPX mimic in the world.Moreover, themutated antibody remains its original antibody titer.

Key words: Glutathione peroxidase, Artificial enzyme, Chemical mutation, Phenylmethyl sulfonyl fluoride, Rabbit Anti-Human IgM(Fcμ)

DING Lan, ZHU Zhen-qi, LUO Gui-min, SUN Qi-an, YANG Tong-shu, SHEN Jia-cong. Studies on Artificial Imitation of Glutathione Peroxidase by Chemical Mutation[J]. Chemical Research in Chinese Universities, 1994, 10(2): 131-133.

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Studies on Artificial Imitation of Glutathione Peroxidase by Chemical Mutation

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