19F NMR Chemical Shift-switching Probes Enabling Simultaneous Detection of Senescence-associated β-Galactosidase and Monoamine Oxidase A

doi:10.1007/s40242-026-5265-3

Abstract

Abstract: ¹⁹F NMR chemical shift-switching probes are highly effective tools for investigating complex biological processes, primarily due to their capacity to produce distinct, crosstalk-free signals from multiple biomarkers. Capitalizing on this capability, we have developed two sets of ¹⁹F NMR probes specifically designed for identifying senescence-associated β-galactosidase (β-gal) and monoamine oxidase A (MAO-A). The probes G-3F and G-F were engineered for β-gal detection, while M-3F, M-2F, and M-F were tailored for MAO-A detection. Among them, probes G-F and M-F demonstrated superior chemical shift changes relative to their series counterparts: G-F's ¹⁹F signal shifted from δ-77.8 to δ -81.8 after reaction with β-gal, while M-F's signal shifted from δ -115.7 to δ -109.2 upon encountering MAO-A. Crucially, the resulting signals are well-separated, which facilitates simultaneous detection without mutual interference, as successfully verified in senescent cell models.

Key words: Molecular probe, ¹⁹F chemical shift, Senescent cell, Biomarker, Simultaneous detection

XIA Ruimin, ZENG Yu, LU Yumeng, WANG Jingyu, LI Ling, LUO Xiangjie, QIAN Yong. ¹⁹F NMR Chemical Shift-switching Probes Enabling Simultaneous Detection of Senescence-associated β-Galactosidase and Monoamine Oxidase A[J]. Chemical Research in Chinese Universities, 2026, 42(2): 470-476.

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¹⁹F NMR Chemical Shift-switching Probes Enabling Simultaneous Detection of Senescence-associated β-Galactosidase and Monoamine Oxidase A

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