Easily Accessible and Highly Selective “Turn-on” Fluorescent Sensor for Imaging Cadmium in Living Cells

doi:10.1007/s40242-013-3126-3

Abstract

Abstract:

A new schiff base of phenothiazine derivative was designed for ratiometric sensing of Cd²⁺ selectively. Upon the addition of Cd²⁺ to the solution of phenothiazine derivative, the fluorescence intensity of it was enhanced in a linear fashion and the maximum fluorescence peak exhibited a blue shift from 575 nm to 525 nm. This ratiometric fluorescent sensor displays a very high sensitivity(detection limits were 0.34 and 1.0 μmol/L of Cd²⁺ using the visual fluorescence changes and UV-Vis changes, respectively), a rapid response time(<10 s) and high selectivity for Cd²⁺ over other transition metal ions. Moreover, the living cells image experiments also demonstrate the value of the sensor in fluorescent visualization of Cd²⁺ in the environmental and biological systems.

Key words: Fluorescent sensor, Blue-shift, Cadmium, Phenothiazine, Cell-imaging

WANG Wei, ZHANG Ying-mu, LI Yao-xian, ZHAO Qing. Easily Accessible and Highly Selective “Turn-on” Fluorescent Sensor for Imaging Cadmium in Living Cells[J]. Chemical Research in Chinese Universities, 2013, 29(4): 632-637.

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