Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (2): 263-269.doi: 10.1007/s40242-013-2430-2

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Fluorosis Caused Cellular Apoptosis and Oxidative Stress of Rat Kidneys

SONG Yang1, WANG Jin-cheng1, XU Hui3, DU Zhen-wu2, ZHANG Gui-zhen2, SELIM Hamid Abdu2, LI Guang-sheng3, WANG Qing2, GAO Zhong-li1   

  1. 1. Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. Central Laboratory, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    3. Pathological Laboratory, Institute of Endemic Disease of Jilin University, Changchun 130021, P. R. China
  • Received:2012-11-12 Revised:2013-01-07 Online:2013-04-01 Published:2013-03-20
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.39730390), the Scientific Research Foundation of Jilin Province of China(No.2008-2123, 20110740, 20100942) and the Health Scientific Research Foundation of Jilin Province of China (No.2010Z083).

Abstract:

As the strongest electronegative element, fluorine can stimulate the production of superoxide radicals in cells. In view of the important roles of kidneys in bone metabolism, the authors analyzed the quantitative pathomorphological characteristics of renal damage and the potential cellular apoptosis and oxidative stress mechanisms in rats treated with excessive fluoride. Wistar rats were exposed to 50 mg F-(110.5 mg NaF)/L, 100 mg F-(221.0 mg NaF)/L and 150 mg F-(331.5 mg NaF)/L in drinking water for 70 and 140 d, respectively. Microscope with image analysis was used to quantitate pathomorphological changes in renal tissues of the rats. Reactive oxygen species(ROS), the cell cycle and apoptosis of renal cells were measured by flow cytometry and TUNEL technique(terminal deoxynucleotidyl transferase dUTP nick end labeling), respectively. The ion concentrations in serum and renal functional parameters were detected by automatic biochemical analyzer. Quantitative analysis results demonstrate the expanded Bowman’s space of glomerulus and obvious dilatation of renal tubule. TUNEL technique revealed that NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3′-indolylphosphate, p-toluidine salt)-staining positive apoptotic cells selectively located in medullocortical junction areas. The data suggest that renal damage in chronic fluorostic rats is associated with the cellular apoptosis and oxidative stress.

Key words: Fluorosis, Apoptosis, Oxidative stress