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Chemical Research in Chinese Universities ›› 2004, Vol. 20 ›› Issue (6): 690-693.

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Electrochemical Determination of Alkaline Phosphatase in Human Serum by Differential Pulse Voltammetry

SUN Wei1,2, JIAO Kui2, WANG Hai-yu2, LU Lu-de1   

  1. 1. College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P. R. China;
    2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China
  • Received:2003-08-19 Online:2004-12-24 Published:2011-08-06
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20075013) and China Postdoctoral Science Foundation(No.2003033492).

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Abstract: Differential pulse voltammetry(DPV) was applied to the determination of alkaline phosphatase(ALP) activity in human serum with phenyl phosphate as the substrate. Phenyl phosphate can enzymatically be hydrolyzed to produce phenol which is quantified by DPV at +660 mV(vs.Ag/AgCl) in the concentration range of 2.0_100 μmol/L. The standard curve for ALP is linear over the range from 0.06 to 1000 U/L with a relative standard deviation of 3.0%. The conditions for the enzymatic reaction and voltammetric detection were optimized and the kinetic constants were also examined.The human serum samples were tested by this method and the results were in good agreement with those obtained by the routine p-nitrophenyl phosphate spectrophotometric method.

Key words: Alkaline phosphatase, Phenyl phosphate, Enzymatic analysis, Differential pulse voltammetry, Human serum sample