A Dual-channel Probe for Detection of O2·- Based on 19F Nuclear Magnetic Resonance and Surface Enhanced Raman Spectroscopy

doi:10.1007/s40242-026-6061-9

Chemical Research in Chinese Universities ›› 2026, Vol. 42 ›› Issue (2): 477-484.doi: 10.1007/s40242-026-6061-9

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A Dual-channel Probe for Detection of O₂^·- Based on ¹⁹F Nuclear Magnetic Resonance and Surface Enhanced Raman Spectroscopy

ZHOU Juyue, JIANG Baian, XU Suying, WANG Leyu

State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, P. R. China

Received:2026-01-15 Online:2026-04-01 Published:2026-04-02
Contact: XU Suying,E-mail:syxu@mail.buct.edu.cn E-mail:syxu@mail.buct.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 22274007, 22334002, 22322402).

Abstract

Abstract: As a key precursor of reactive oxygen species (ROS), the superoxide anion (O₂^·^-) plays a pivotal role in maintaining redox homeostasis in living systems. However, the accurate and reliable detection of O₂^·^- remains a significant challenge, particularly for in vivo detection. Herein, we report a dual-channel probe for O₂^·^- detection based on ¹⁹F nuclear magnetic resonance (¹⁹F NMR) and surface-enhanced Raman scattering (SERS) spectroscopy. Through structure engineering, the molecular probe (FS) was designed, which selectively reacted with O₂^·^-, generating a characteristic chemical shift in ¹⁹F NMR. Notably, the fragment released upon reaction with O₂^·^- serves as an effective SERS reporter, thereby enabling complementary dual-channel sensing of superoxide anion. Furthermore, as a proof-of-concept, probe FS was explored as a synthetic biomarker for urinalysis to detect O₂^·^- generated in vivo and found clear different metabolic patterns between healthy and liver-injured models, suggesting its potential for in vivo detection.

Key words: ¹⁹F nuclear magnetic resonance (NMR), Surface-enhanced Raman scattering (SERS), O₂^·- detection, Urinalysis

ZHOU Juyue, JIANG Baian, XU Suying, WANG Leyu. A Dual-channel Probe for Detection of O₂^·- Based on ¹⁹F Nuclear Magnetic Resonance and Surface Enhanced Raman Spectroscopy[J]. Chemical Research in Chinese Universities, 2026, 42(2): 477-484.

References

[1] Xu T., Ji W., Zhang Y., Wang X., Gao N., Mao L., Zhang M., Angew. Chem. Int. Ed., 2022, 61, e202204344.
[2] Li X., Dunevall J., Ewing A. G., Acc. Chem. Res., 2016, 49, 2347.
[3] Fan H., Sasaki Y., Zhou Q., Tang W., Nishina Y., Minami T., Chem. Commun., 2023, 59, 2425.
[4] Watts S. W., Gilbert L., Webb R. C., Hypertension, 1995, 26, 1056.
[5] Chechekhin V. I., Ivanova A. M., Kulebyakin K. Y., Antropova Y. G., Karagyaur M. N., Skryabina M. N., Chechekhina E. S., Basalova N. A., Grigorieva O. A., Sysoeva V. Y., Kalinina N. I., Tkachuk V. A., Tyurin-Kuzmin P. A., Biochim. Biophys. Acta, Mol. Cell Res., 2024, 1871, 119651.
[6] Pourhamzeh M., Moravej F. G., Arabi M., Shahriari E., Mehrabi S., Ward R., Ahadi R., Joghataei M. T., Cell. Mol. Neurobiol., 2022, 42, 1671.
[7] Archambault J. L., Delaney C. A., Biomedicines, 2023, 11, 3049.
[8] Missouris C. G., Cappuccio F. P., Varsamis E., Barron J. L., Carr E., Markandu N. D., MacGregor G. A., Am. Heart J., 1998, 135, 838.
[9] Atici T., Bilgi Kamaç M., Yilmaz M., Yilmaz Kabaca A., Electrochim. Acta, 2023, 458, 142484.
[10] Wang Y., Gu C., Patel B. A., Ewing A. G., Angew. Chem. Int. Ed., 2021, 60, 23552.
[11] Phung V.-D., Kook J.-K., Koh D.Y., Lee S.-W., Dalton T., 2019, 48, 16026.
[12] Qiao X., Su B., Liu C., Song Q., Luo D., Mo G., Wang T., Adv. Mater., 2018, 30, 1702275.
[13] Qian Y., Zhang L., Tian Y., Anal. Chem., 2022, 94, 1447.
[14] Dieringer J. A., Lettan R. B., Scheidt K. A., Van Duyne R. P., J. Am. Chem. Soc., 2007, 129, 16249.
[15] Li P., Ge B., Ou L. M.-L., Yao Z., Yu H.-Z., Sensors, 2015, 15, 20543.
[16] Zhang T., Quan X., Cao N., Zhang Z., Li Y., Nanomaterials, 2022, 12, 3199.
[17] Zhou X., Hu Z., Yang D., Xie S., Jiang Z., Niessner R., Haisch C., Zhou H., Sun P., Adv. Sci., 2020, 7, 2001739.
[18] Mei R., Wang Y., Yu Q., Yin Y., Zhao R., Chen L., ACS Appl. Mater. Interfaces, 2020, 12, 2059.
[19] Shi L., Liu M., Zhang L., Tian Y., Angew. Chem. Int. Ed., 2022, 61, e202117125.
[20] Zhao Y., Shi L., Tian Y., Zhang L., Chin. J. Chem., 2023, 41, 569.
[21] Lin D., Gong T., Qiu S., Wu Q., Tseng C. Y., Kong K. V., Chen G., Chen R., Chem. Commun., 2019, 55, 1548.
[22] Prashanth S., Aziz R. A., Raghu S. V., Shim Y.-B., Sudhakara Prasad K., Adhikari A. V., Mater. Adv., 2024, 5, 1185.
[23] Wang H., Jiang X., He Y., Analyst, 2016, 141, 5010.
[24] Nakatsuka N., Yang K.-A., Abendroth J. M., Cheung K. M., Xu X., Yang H., Zhao C., Zhu B., Rim Y. S., Yang Y., Weiss P. S., Stojanović M. N., Andrews A. M., Science, 2018, 362, 319.
[25] Nakatsuka N., Faillétaz A., Eggemann D., Forró C., Vörös J., Momotenko D., Anal. Chem., 2021, 93, 4033.
[26] Kanda K., Adachi O., Kawatsu S., Sakatsume K., Kumagai K., Kawamoto S., Saiki Y., J. Thorac. Cardiovasc. Surg., 2016, 152, 1401.
[27] Wan Y., Guo Z., Jiang X., Fang K., Lu X., Zhang Y., Gu N., J. Colloid Interface Sci., 2013, 394, 263.
[28] Wang Y., Li Y., Yang X., Wang T., Du X., Zhu A., Xie W., Xie W., Angew. Chem. Int. Ed., 2024, 63, e202318817.
[29] Van De Werd H. J., Uylings H. B., Brain Struct. Funct., 2014, 219, 433.
[30] Alzoubi F. Y., Ahmad A. A., Aljarrah I. A., Migdadi A. B., Al-Bataineh Q. M., J. Mater. Sci. Mater. Electron., 2023, 34, 2128.
[31] El-Zahry M. R., Talanta, 2018, 186, 229.
[32] Zlotnikov I. D., Streltsov D. A., Ezhov A. A., Kudryashova E. V., Pharmaceutics, 2023, 15, 1135.
[33] Buford T. W., Ageing Res. Rev., 2016, 26, 96.
[34] Zhang F., Yang L., Wei J., Tian X., Sensors, 2023, 24, 238.
[35] Gulati A., Arora R. C., Crayton J., Eur. J. Pharmacol., 1993, 231, 151.
[36] Kermorgant M., Lancien F., Mimassi N., Le Mével J.-C., Respir. Physiol. Neurobiol., 2014, 192, 55.
[37] Watts S. W., Davis R. P., Cardiovasc. Ther., 2011, 29, 54.

A Dual-channel Probe for Detection of O₂^·- Based on ¹⁹F Nuclear Magnetic Resonance and Surface Enhanced Raman Spectroscopy

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