CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (3): 472-476.

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Wound Healing Assay Using Microfluidic Channels

XI Yan-li1, WANG Shu-tao2, SU Bin2, HA Qing1, NIU Feng-lan1, NIE Fu-qiang2   

  1. 1. School of Public Health, Jinlin University, Changchun 130021, P. R. China;
    2. Beijing National Laboratory for Molecular Sciences(BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China
  • Received:2011-11-23 Revised:2012-02-02 Online:2012-05-25 Published:2012-05-03
  • Contact: NIU Feng-lan, NIE Fu-qiang E-mail:niufl@jlu.edu.cn; fqnie@iccas.ac.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.21071148, 21175140).

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Abstract: An approach was described to produce a wound area in vitro using laminar flow technique to selectively remove cells in microfluidic channels. Cell migration which plays an important role in the process of wound healing can be observed dynamically by this method. In order to prove our system, we have studied its properties by injecting NIH-3T3 cells into main channel and added several reagents to observe the mediatory influence. The results reveal that fibroblast growth factor-2(FGF-2), insulin-like growth factor 1(IGF-I), platelet-derived growth factor BB(PDGF-BB) and ascorbic acid(Vc) groups can promote cell migration compared with the control group. But the average migration distance was diminished in dexamethasone group. It can be concluded that this system can be used to analyze the process of wound healing.

Key words: Wound healing, Cell migration, Microfluidic channel