CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (3): 454-458.

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Antioxidant Effect of Selenium-containing Glutathione S-Transferase in Rat Cardiomyocytes

YIN Li1, HAN Xiao1, YU Yang1, GUO Xiao1, REN Li-qun1, FANG Jing-qi1, LIU Zhi-yi1, YAN Gang-lin2, WEI Jing-yan1   

  1. 1. College of Pharmaceutical Science, Jilin University, Changchun 130021, P. R. China;
    2. Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, College of Life Science, Jilin University, Changchun 130012, P. R. China
  • Received:2011-12-26 Revised:2012-02-08 Online:2012-05-25 Published:2012-05-03
  • Contact: YAN Gang-lin, WEI Jing-yan E-mail:jingyanwei@yahoo.com.cn; ygl@jlu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30870540, 30970633).

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Abstract: As one of the most important antioxidant enzymes, glutathione peroxidase(GPX) protects cells and tissues from oxidative damage, and plays an important role in cardiovascular and cerebrovascular injuries induced by oxidative stress. The antioxidant effect of selenium-containing glutathione S-transferase(Se-GST), a mimic of GPX was investigated on rat cardiomyocytes. To explore the protection function of Se-GST in hydrogen peroxide(H2O2) challenged rat cardiomyocytes, we examined malondialdehyde(MDA), lactate dehydrogenase(LDH), superoxide dismutase( SOD) and cell apoptosis. The results demonstrate exposure of rat cardiomyocytes to H2O2 for 6 and 12 h induced the significant increases of MDA, LDH and apoptosis rate of cardiomyocytes, but pretreatment of rat cardiomyocytes with Se-GST at 0.0005 or 0.001 unit/mL prevents oxidative stress induced by H2O2 with the decreases of cell apoptosis. All the results hint Se-GST has antioxidant activity for oxidative stress challenged rat cardiomyocytes.

Key words: Selenium-containing glutathione S-transferase(Se-GST), Cell apoptosis, Cardiac myocyte, Oxidative stress