CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (3): 449-453.

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Antitumor Active Protein-containing Glycans from the Body of Ganoderma tsugae

LIU Ying1,3, LI Yue-fei4, ZHENG Ke-yan2, FEI Xiao-fang1   

  1. 1. College of Life Science, Jilin University, Changchun 130012, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
    3. Department of Cosmetology, Affiliated Hospital, Changchun University of Traditional Chinese Medicine, Changchun 130021, P. R. China;
    4. Department of Osteology, Affiliated Hospital, Changchun University of Traditional Chinese Medicine, Changchun 130021, P. R. China
  • Received:2012-01-16 Revised:2012-03-31 Online:2012-05-25 Published:2012-05-03
  • Contact: FEI Xiao-fang E-mail:feixf@jlu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30870552).

PDF (PC)

Abstract: To explore the effects of traditional herbal medicine Ganoderma tsugae(G. tsugae) on immunomodulatory and antitumor activities, the crude polysaccharides of G. tsugae were purified by filtration, diethylaminoethyl(DEAE) sepharose-fast flow chromatography and sephadex G-100 size-exclusion chromatography. Two main fractions, protein- containing glycans CSSLP-1 and CSSLP-2, were obtained via the gradient elution. The protein content, molecular weight, and monosaccharide composition of the two fractions were analyzed. Furthermore, the influence of the protein-containing glycans from G. tsugae on the activation of human acute monocytic leukemia cell line(THP-1) and their antitumor activities to the human hepatocellular liver carcinoma cell(HepG-2) in vitro were evaluated. The results indicate that CSSLP-1 and CSSLP-2 could increase the pinocytic activity of THP-1 cells and induce THP-1 cells to produce the cytokines of TNFα and IL-2, significantly. CSSLP-1 and CSSLP-2 also played an inhibiting effect on the cancer cell(HepG-2). Moreover, the anti-proliferation activity of CSSLP-1 and CSSLP-2 increased with the participation of TNFα and IL-2 or other antitumor factors induced from THP-1 cells by G. tsugae protein-containing glycan fractions.

Key words: Protein-containing glycan, Ganoderma tsugae, Antitumor activity