Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (2): 353-357 .

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Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

School of Materials Science and Mechanical Engineering, Beijing Technology and Business University, Beijing 100048, P. R. China

Received:2011-07-01 Revised:2011-09-10 Online:2012-03-25 Published:2012-03-08
Supported by:
Supported by the Scientific and Technological Development Programs of Beijing Municipal Education Commission, China (No.KM201010011004), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education, China, the 2011 Merit-based Research Funding for Picked Returned Overseas Scholars, Beijing Municipality(China) and the Research Project Approved for Graduate Students of Beijing Technology and Business University, China(No.19000101026).

Abstract

Abstract: UV-induced graft polymerization of acrylic acid(AA) on poly(ether ether ketone)(PEEK) films was carried out to introduce ―COOH for the subsequent immobilization of bovine serum albumin(BSA). BSA was introduced on PEEK surface based on the condensation reaction between ―NH₂ and ―COOH. The modified surface (PEEK-BSA) was characterized by energy-disperse spectrometry(EDS), X-ray photoelectron spectroscopy(XPS), water contact angle measurement and UV spectrum analysis. The contact angle was found to decrease from 104° for the virgin PEEK films to 63° for the BSA-immobilized PEEK films, demonstrating a significant improvement of surface hydrophilicity. Moreover, the appearance of nitrogen on PEEK film confirmed by XPS and EDS indicates the immobilization of BSA on PEEK surface.

Key words: Poly(ether ether ketone)(PEEK), UV-grafting, Bovine serum albumin immobilization

SUN Hui, CHEN Rui-chao, LI Ang, XU Guo-zhi. Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement[J]. Chemical Research in Chinese Universities, 2012, 28(2): 353-357 .

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Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

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