Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (1): 103-107.

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Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

ZHU Chun-yu^1,2, SUN Ting-ting², ZHAO Jian^1,2, WANG Ning^1,2, ZHENG Fang-liang¹, AI Hai-xin¹, ZHU Jun-feng^1,2, WANG Xiao-ying³, ZHU Ying⁴, WU Jian-guo⁴, LIU Hong-sheng^1,2*

1. Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province;
2. Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province, Academy of Science, Liaoning University, Shenyang 110036, P. R. China;
3. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, P. R. China;
4. State Key Laboratory of Virology, Wuhan University, Wuhan 430072, P. R. China

Received:2011-05-10 Revised:2011-08-24 Online:2012-01-25 Published:2011-12-27
Contact: hongsheng liu E-mail:liuhongsheng@lnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.30671852), the Open Research Fund Program of the State Key Laboratory of Virology of China(Nos.2010009, 2007007) and the Research Fund of the Key Laboratory of Department of Education of Liaoning Province, China(No.2009S043).

Abstract

Abstract: Avian influenza virus(AIV) nonstructural 1(NS1) gene was amplified by real-time polymerse chain reaction( RT-PCR) and inserted into pET28a, then transformed into E. coli BL21(DE3) competent cell. With the induction of isopropyl-β-D-thiogalactoside(IPTG) and the purification of Ni-NTA column, we finally obtained purified NS1 protein. T7-phage display system was used to screen the proteins that interacted with NS1 from lung cell cDNA library. The selected positive clones were identified by DNA sequencing and analyzed by BLAST program in Gene- Bank. Two proteins were obtained as NS1 binding proteins, Homo sapiens nucleolar and coiled-body phosphoprotein 1(NOLC1) and Homo sapiens similar to colon cancer-associated antigen. By co-immunoprecipitation and other methods, Homo sapiens NOLC1 was found to interact with the NS1 protein, the results would provide the basis for further studying biological function of NS1 protein.

Key words: T7-phage display, Nonstructural 1(NS1) protein, Interacting protein

ZHU Chun-yu, SUN Ting-ting, ZHAO Jian, WANG Ning, ZHENG Fang-liang, AI Hai-xin, ZHU Jun-feng, WANG Xiao-ying, ZHU Ying, WU Jian-guo, LIU Hong-sheng. Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library[J]. Chemical Research in Chinese Universities, 2012, 28(1): 103-107.

References

[1] Takeyama N., Minari K., Kajihara M., Isoda N., Sakamoto R., SasakiT., Kokumai N., Takikawa N., Shiraishi R., Mase M., Hagiwara J.,Kodama T., Imamura T., Sakaguchi M., Ohgitani T., Sawata A.,Okamatsu M., Muramatsu M., Tsukamoto K., Lin Z., Tuchiya K.,Sakoda Y., Kida H., Veterinary Microbiology, 2011, 147(3/4), 283 [2] Zhao L., Xu L., Zhou X., Zhu Q., Yang Z., Zhang C., Zhu X., Yu M.,Zhang Y., Zhao X., Huang P., Virology Journal, 2009, 6(1), 218 [3] Jiao P., Tian G., Li Y., Deng G., Jiang Y., Liu C., Liu W., Bu Z.,Kawaoka Y., Chen H. A., Journal of Virology, 2008, 82(3), 1146 [4] Mitrasinovic P. M., Biophysical Chemistry, 2009, 140(1―3), 35 [5] Zhao Z. M., Shortridge K. F., Garcia M., Guan Y., Wan X. F.,Journal of General Virology, 2008, 89(9), 2182 [6] Monto A. S., The New England Journal of Medicine, 2005, 352(4),323 [7] Kwon Y. K., Lipatov A. S., Swayne D. E., Veterinary Pathology,2009, 46(1), 138 [8] Tumpey T. M., Alvarez R., Swayne D. E., Suarez D. L., Journal of Clinical Microbiology, 2005, 43(2), 676 [9] Better M., Chang C. P., Robinson R. R., Horwitz A. H., Science,1988, 240(4855), 1041 [10] Mangeney M., Parseval N., Thomas G., Heidmann T., Journal of Genetic Virology, 2001, 82(10), 2515 [11] Crameri R., Jaussi R., Menz G., Blaser K., European Journal of Biochemistry,1994, 226(1), 53 [12] Rosenberg A., Griffin K., Studier W., Mccormick M., Berg J., Novy R., Mierendorf R., Novations Novagen Newsletter, 1996, 637(6), 63 [13] Jung S., Honegger A., Pluckthun A., Journal of Molecular Biology,1999, 294(1), 163 [14] Liu Z. Y., Wang H. S., Zhang L. Y., Cheng J., World Chinese Journal of Digestology, 2007, 15(27), 2914 [15] Jin Y., Yu J., Yu Y. G., Chemistry and Biology, 2002, 9(2), 157 [16] Wolff T., ONeill R. E., Palese P., Journal of Virology, 1998, 72(9),7170 [17] Aragon T., Luna S., Novoa I., Carrasco L., Ortin J., Nieto A., Molecularand Cellular Biology, 2000, 20(17), 6259 [18] Bergmann M., Garcia-Sastre A., Carnero E., Pehamberger H., WolffK., Palese P., Muster T., Journal of Virology, 2003, 74(13), 6203 [19] Campanini G., Piralla1A., Paolucci1 S., Rovida F., Percivalle E.,Maga G., Baldanti F., Journal of Virology, 2010, 7, 209 [20] Huang Y., Cai X. F., Zhang J., Huang A. L., Acta Academine Medicine Militaris Tertiae, 2007, 29(10), 876

Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

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