Investigation on the Photodissociation of Oxygen from Oxymyoglobin by Fluorescence Spectroscopy

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (6): 1060-1064.

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Investigation on the Photodissociation of Oxygen from Oxymyoglobin by Fluorescence Spectroscopy

ZHOU Hua-wei¹, CAO Hong-yu^1,2, TANG Qian^1,2, MA Jun-yan², ZHANG Ying-ying² and ZHENG Xue-fang^1,2*

1. College of Bioengineering,
2. Liaoning Key Lab of Bio-organic Chemistry, Dalian University, Dalian 116622, P. R. China

Received:2010-10-12 Revised:2011-04-07 Online:2011-11-25 Published:2011-11-07
Contact: ZHENG Xue-fang E-mail:dlxfzheng@126.com
Supported by:
Supported by the National Natural Science Foundation of China(No.20871024), the Program for Liaoning Excellent Talents in University, China(No.RC-04-10), the Fund of Liaoning Innovative Research Team in University, China(Nos.2006T002, 2008T005, 2009T003), and the Plan Project of Dalian Science and Technology, China(No.2008E11SF170).

Abstract

Abstract: Photodissociation of oxygen from oxymyoglobin(oxyMb) was investigated by means of fluorescence spectroscopy. One of the most important findings of the photodissociation of oxyMb was the discovery of two processes which were affected by excitation intensity, temperature, solvent viscosity, and excitation wavelength. Process I(PI) corresponded to oxygen escaping from the binding site at ferrous heme iron atom within the porphyrin ring into the heme pocket, whereas process II(PII) was ascribed to oxygen escaping from the heme pocket into the solvent. To elucidate this interesting phenomenon, we proposed a model that oxygen encountered two barriers on its way from the binding site at the ferrous heme iron to the solvent. Reversibility and wavelength sensitivity of the photodissociation were also observed.

Key words: Oxymyoglobin, Photodissociation, Escaping pathway, Barrier, Fluorescence spectroscopy

CLC Number:

ZHOU Hua-wei, CAO Hong-yu, TANG Qian, MA Jun-yan, ZHANG Ying-ying and ZHENG Xue-fang*. Investigation on the Photodissociation of Oxygen from Oxymyoglobin by Fluorescence Spectroscopy[J]. Chemical Research in Chinese Universities, 2011, 27(6): 1060-1064.

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Investigation on the Photodissociation of Oxygen from Oxymyoglobin by Fluorescence Spectroscopy

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