CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (6): 1000-1005.

• Articles • Previous Articles     Next Articles

Novel Type II Peroxiredoxin Gene Homologue from Chinese Wildrye Enhancing Salt Stress Tolerance of Transgenic Yeast

YU Ying1, LIU Xiang-guo2, LU Yang1, DOU Yao1, WANG Hu-yi1, HAN Si-ping2, FENG Shu-dan3 and HAO Dong-yun1,2*   

  1. 1. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130012, P. R. China;
    2. Biotechnology Research Centre, Jilin Academy of Agricultural Sciences, Changchun 130033, P. R. China;
    3. College of Life Science and Technology, Harbin Normal University, Harbin 150080, P. R. China
  • Received:2011-01-13 Revised:2011-03-09 Online:2011-11-25 Published:2011-11-07
  • Contact: HAO Dong-yun E-mail:dyhao@cjaas.com
  • Supported by:

    Supported by the Transgenic Plant Research Special Program of China(No.2008ZX08003-005) and the Technology Deve- lopment Project of Jilin Province, China(Nos.20086029, 20076016).

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Abstract: Peroxiredoxins(Prxs) are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species(ROS). Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye(Leymus Chinensis), designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H2O2 and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na2CO3 in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na2CO3.

Key words: Chinese wildrye, Monocot, Salt stress, Type II peroxiredoxin