CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (4): 646-650.

• Articles • Previous Articles     Next Articles

Anti-tumor Effects of a Recombinant Fowlpox Virus Expressing Apoptin on Human Cervical Carcinoma in Vivo and in Vitro

ZHU Ji-hong1,2, LI Xiao2, SUN Li-li2,3, ZHANG Mu-chun2,4, KAN Shi-fu2, LIU Lei2,5, HUANG Hai-yan2,5, YANG Guo-hua2,5, PIAO Bing-guo2,5 and JIN Ning-yi2*   

  1. 1. Department of Gynecology and Obstetrics, the First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, P. R. China;
    3. Department of Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun 130012, P. R. China;
    4. Department of Urology, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    5. Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, P. R. China
  • Received:2010-06-28 Revised:2010-09-16 Online:2011-07-25 Published:2011-06-29
  • Contact: JIN Ning-yi E-mail:ningyik@126.com
  • Supported by:

    Supported by the National High-Tech Research and Development Program of China(No.2007AA021004), the National Basic Research Program of China(No.2005CB523005), the National Science and Technology Major Project of China (Nos.2008ZX10004-015, 2009ZX08006-002B) and the National Natural Science Foundation of China(No.30771609).

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Abstract: Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells, but not of normal diploid cells. To explore the application of apoptin in tumor gene therapy, we used a recombinant fowlpox virus expressing apoptin protein (vFV-Apoptin) to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma(HeLa) cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT) assay, acridine orage/ethidium bromide(AO/EB) and annexin V staining test, respectively. The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells, and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship. The results of animal models show that vFV-Apoptin significantly inhibits tumor growth, extends the lifespan of animals and improves the mean survival. Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy.

Key words: Apoptin gene, Chicken anemia virus, Human cervical carcinoma, Anti-tumor effect