CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (3): 464-467.

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Expression of SOCS-3 and Its Methylation in Renal Cancer

YU Jing1, ZHAO Li-chun2, YAO Song-mei1, HE Cheng-yan1, LI Hong-jun1* and KONG Xiang-bo1   

  1. 1. China-Japan Union Hospital,
    2. College of Pharmacy, Jilin University, Changchun 130033, P. R. China
  • Received:2010-11-17 Revised:2011-01-06 Online:2011-05-25 Published:2011-04-29
  • Contact: LI Hong-jun E-mail:lihongjun1960@126.com
  • Supported by:

    Supported by the Thirty-ninth Postdoctoral Science Foundation of China(No.20060390907).

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Abstract: The expression level of suppressor of cytokine signaling-3(SOCS-3) in human renal carcinoma and its methylation state were investigated. Reverse transcription-polymerase chain reaction(RT-PCR), immuocytochemistry, immunohistochemistry and Western blot were used to detect the expression level of SOCS-3, and the methylation of SOCS gene was investigated by methylation specific PCR in the tissues of 15 cases of renal carcinoma. Compared to those of the normal renal cell line and specimens , the expression level of SOCS-3 in renal carcinoma was significantly lower or can’t be detected(P<0.01). And the methylation of SOCS-3 gene in the tissue of renal carcinoma was significantly higher. The expression of SOCS-3 gene is significantly lower in renal carcinoma and the high methylation of the promoter island of SOCS-3 gene is associated with the lower expression of SOCS-3 gene. It may be one of main mechanisms for the development and progress of renal carcinoma.

Key words: Renal carcinoma, Suppressor of cytokine signaling(SOCS), DNA methylation