Regulating Effect of Allicin(Diallyldisulfid-S-Oxide) on Respiration Function of Mitochondria from Beef Heart

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (5): 785-791.

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Regulating Effect of Allicin(Diallyldisulfid-S-Oxide) on Respiration Function of Mitochondria from Beef Heart

SU Yan-bin1,2, TONG Li-fang3, LIU Pai1, LU Ming1, CHENG Ying-kun1, ZHANG Feng-rong4, SU Yan-wen2, DONG Jia-ning1 and LI Zheng-qiang1*

1. Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Jilin University, Changchun 130021, P. R. China;
2. School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, P. R. China;
3. Department of Infective Disease Therapeutics of China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China;
4. Jilin Provincial Institute for Drug and Food Control, Changchun 130033, P. R. China

Received:2010-03-19 Revised:2010-08-23 Online:2010-09-25 Published:2010-11-26
Contact: LI Zheng-qiang. E-mail: lzq@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.30570404 and 30670458).

Abstract

Abstract: To probe into regulating functions of allicin, diallyldisulfid-S-oxide, on electron transport in the inner membrane of mitochondria isolated from beef heart, under the anaerobic, hypoxia, and oxygenating conditions in the presence of rotenone(complex I inhibitor) at 37 °C, we optimized the isolation method for isolating mitochondria from beef heart and used the technique for measuring dissolved oxygen content at the right moment so as to investigate the effects of allicin on the respiratory control of mitochondria in state 4 and state 3, P/O ratio, and respiratory control ratio(RCR). The results show that the optimized isolated requirements were that all the operations were always kept at 0―4 °C, the ratio between sample and isolation medium was 1:4, the filter was a four-layer gauze, two centrifugations in different sections. First section: 2500g, 10 min; second section 7000g, 20 min. The results indicate that the capacity of dissolved oxygen in state 3 was increased and that of dissolved oxygen in state 4 was almost stabilized in mitochondria from beef heart.

Key words: Allicin, Respiration, Mitochondrial, Beef heart

SU Yan-bin1,2, TONG Li-fang3, LIU Pai1, LU Ming1, CHENG Ying-kun1, ZHANG Feng-rong4, SU Yan-wen2, DONG Jia-ning1 and LI Zheng-qiang1*. Regulating Effect of Allicin(Diallyldisulfid-S-Oxide) on Respiration Function of Mitochondria from Beef Heart[J]. Chemical Research in Chinese Universities, 2010, 26(5): 785-791.

References

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Regulating Effect of Allicin(Diallyldisulfid-S-Oxide) on Respiration Function of Mitochondria from Beef Heart

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