Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh2 on Hep-A-22 Cells

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (4): 567-571.

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Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh₂ on Hep-A-22 Cells

ZHOU Hong-yu¹, JIN Yong-ri¹, WEI Wei¹, SHI Xiao-lei¹, YANG Rui-jie¹, YANG Shi-jie² and LI Xu-wen^1*

1. College of Chemistry, Jilin Uuniversity, Changchun 130012, P. R. China;
2. Basic Medical School, Jilin Uuniversity, Changchun 130021, P. R. China

Received:2009-08-31 Revised:2009-12-29 Online:2010-07-25 Published:2010-10-01
Contact: LI Xu-wen. E-mail: xwli@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.30672654).

Abstract

Abstract: A modified method of preparing 20(S)-ginsenoside Rh₂(G-Rh2) and the inhibitory effect of 20(S)-ginse- noside Rh₂ on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh₂ had been isolated and purified, MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh₂ on the cells viability and morphological changes were observed. It was shown that 20(S)-ginsenoside Rh₂ can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it. In conclusion, 20(S)-ginsenoside Rh₂ can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.

Key words: 20(S)-Ginsenoside Rh2, Preparation, Alkaline-degradation, Hep-A-22 cell, Inhibitory effect

ZHOU Hong-yu, JIN Yong-ri, WEI Wei, SHI Xiao-lei, YANG Rui-jie, YANG Shi-jie,.... Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh₂ on Hep-A-22 Cells[J]. Chemical Research in Chinese Universities, 2010, 26(4): 567-571.

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Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh₂ on Hep-A-22 Cells

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