Rapid Profiling of Alkaloids in Several Medicinal Herbs by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (1): 11-16.

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Rapid Profiling of Alkaloids in Several Medicinal Herbs by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

LU Lin^1,3, YUE Hao¹, SONG Feng-rui¹, TSAO Rong², LIU Zhi-qiang^1* and LIU Shu-ying¹

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, N1G5C9, Canada;
3. Graduate School, Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2009-08-27 Revised:2009-11-11 Online:2010-01-04 Published:2010-03-29
Contact: LIU Zhi-qiang. E-mail: liuzq@ciac.jl.cn
Supported by:
Supported by the Major State Basic Research Development Program of China(No.2006CB5047060), the National Natural Science Foundation of China(Nos.30672600, 30772721) and the Natural Science Foundation of Jilin Province, China (No.20060902).

Abstract

Abstract:

A simple method was developed for rapid and direct profiling of alkaloids in medical herbs via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). The dry herbs were first ground to powder and passed through a stainless steel sieve, mixed with the matrix solution to form a homogeneous suspension, which was then directly applied to MALDI analysis. Several matrices were investigated and 2,5-dihydroxybenzoic acid(DHB) was chosen as the optimized one, and the particle with small size was found to favor the analysis. Using this method, the profiles of alkaloids in several medical herbs were readily obtained, and the toxicities of crude and processed Radix Aconiti Lateralis Preparata were compared via the relative intensities of the peaks of the corresponding toxic components shown in their MALDI spectra. This method therefore provides a rapid and reliable protocol for obtaining profiles of alkaloids in medical herbs by using MALDI-TOF MS.

Key words: Matrix-assisted laser desorption/ionization; Alkaloid; Medical herb

LU Lin, YUE Hao, SONG Feng-rui, TSAO Rong, LIU Zhi-qiang* and LIU Shu-yin. Rapid Profiling of Alkaloids in Several Medicinal Herbs by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry[J]. Chemical Research in Chinese Universities, 2010, 26(1): 11-16.

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Rapid Profiling of Alkaloids in Several Medicinal Herbs by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

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