Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (6): 899-903.

• Articles • Previous Articles     Next Articles

Effects of Solvents on Extraction of Effective Components from Cell Wall Tissues of Chinese Traditional Medicines

LIU Cheng-bai1, TIAN Luo1, YU Zhen-xiang2, ZHANG Feng-chun1 and CHEN Xia1*   

  1. 1. College of Life Science, Jilin University, Changchun 130021, P. R. China;
    2. Department of Respiratory Medicine, First Hospital of Jilin University, Changchun 130021, P. R. China
  • Received:2009-03-04 Revised:2009-06-28 Online:2009-11-25 Published:2010-01-25
  • Contact: CHEN Xia. E-mail: bbbbz@163.com
  • Supported by:

    Supported by the Major Program of Chinese Herbal Modernism Science of Jilin Provincial Science and Technology Department, China(No. 20030906-01).

Abstract:

The effects of different solvents on the extraction of effective components from the cell wall tissues of Chinese traditional medicines were studied by the histochemical methods, such as the bare-handed section, swelling ratio, paraffin section, IR spectrum and cell wall component analysis. The results show that the ethanol-alkali solvent could increase the swelling ratio as well as the swelling speed. The effective components of cell wall tissues extracted by ethanol-alkali solvent become loose shown by the paraffin section. According to the IR spectrum analysis and the results of cell wall tissue component analysis, it was found that the ethanol-alkali solvent could decrease the contents of pectin and hemicellulose in the cell wall to make the wall broken, and therefore the effective components can be extracted easily by the solvent and the extraction rate is increased.

Key words: Solvent; Cell wall; Paraffin Section; Infrared spectrometry