Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (6): 882-886.

• Articles • Previous Articles     Next Articles

Construction of Saccharomyces cerevisiae Strain Stably Expressing a Fusion Protein Containing Ten Tandem Recombinant Human Glucagon-like Peptide-1 Analogues

WU Zhi-qiang1, JIA Nai-bing1, LI Na2, MA Bai-cheng1 and LI Ming-gang1*   

  1. 1. Institute for Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, P. R. China;
    2. Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300201, P. R. China
  • Received:2008-10-07 Revised:2008-12-01 Online:2009-11-25 Published:2010-01-25
  • Contact: LI Ming-gang. E-mail: mgl@nankai.edu.cn

Abstract:

The recombinant Saccharomyces cerevisiae strain stably expressing recombinant human glucagon-like peptide-1(rhGLP-1) analogue, as a potential oral drug delivery system for diabetes type II treatment, was successfully constructed by the homologous recombination between chromosomal DNA and yeast and integrating vector pNK-GLP containing yeast ribosomal DNA fragments. The amount of rhGLP-1 analogue fusion protein in transformant SG2 reached ca. 0.84 mg per gram of packed cells when SG2 was grown for 24 h in the YPD medium with a inoculum and medium ratio of 1:1. Oral administration of 5 g lyophilized SG2/kg to hyperglycemic rats decreased serum glucose from (24.8±1.40) to (21.2±1.36) mmol/L.

Key words: Saccharomyces cerevisiae; Yeast integrating vector; Ribosomal DNA; Recombinant human glucagon-like peptide-1; Hyperglycemic rat