Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (6): 817-821.

• Articles • Previous Articles     Next Articles

Isolation and Purification of Plantamajoside and Acteoside from Plant Extract of Plantago asiatica L. by High Performance Centrifugal Partition Chromatography

LI Li1, LIU Chun-ming1*, CHEN Zhao-jie3, WANG Jing1, SHI Dong-fang1 and LIU Zhi-qiang2   

  1. 1. The Central Laboratory, Changchun Normal University, Changchun 130032, P. R. China;
    2. Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130021, P. R. China;
    3. Department of Endocrinology of China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China
  • Received:2008-12-23 Revised:2009-04-22 Online:2009-11-25 Published:2010-01-25
  • Contact: LIU Chun-ming. E-mail: chunmingliu2000@yahoo.com.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30873364) and the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167).

Abstract:

Two phenylethanoid glycosides(PhGs), plantamajoside and acteoside, were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography(HPCPC) with ethyl acetate, n-butanol, ethanol and water(0.5:0.5:0.1:1, volume ratio) as solvent system. A total of 45.6 mg of  plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of >93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry(ESI-MSn) in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.

Key words: Plantago asiatica L; Plantamajoside; Acteoside; HPCPC; ESI-MSn; HPLC-DAD