Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (4): 556-559.

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Interaction Between 1,2,3-Trichloropropane and Haloalkane Dehalogenase LinB

ZHAO Yong-shan, ZHENG Qing-chuan*, ZHANG Hong-xing, CHU Hui-ying and SUN Chia-chung   

  1. State Key Laboratory of Theoretical and Computational Chemistry, Institute of Theoretical Chemistry, Jilin University, Changchun 130021, P. R. China
  • Received:2009-03-23 Revised:2009-05-13 Online:2009-07-25 Published:2009-10-16
  • Contact: ZHENG Qing-chuan. E-mail: zhengqch@jlu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20573042), Key Projects in the National Science & Technology Pillar Program of China(No.2006BAE03B01), Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20070183046), and Specialized Fund for the Basic Research of Jilin University, China(No.200810018).

Abstract:

The haloalkane dehalogenase LinB from Sphingomonas paucimobills UT26 was found to transform the 1,2,3-trichloropropane(TCP) into inorganic halide ions and 2,3-dichloro-1-propanol although the catalytic activity is very low(Kcat=0.005 s–1). In this study, molecular dynamics simulation and docking studies were performed to investigate the binding of TCP to LinB. The docking results indicate that LinB does not restrict TCP to be bound productively in the active site and the water-mediated inhibition occurs in the process of TCP interacting with LinB. The residues Ile134, Leu150, Phe154, Pro208, and Ile211 located on the cap domain are potential targets for mutagenesis researches.

Key words: Haloalkane dehalogenase; 1,2,3-Trichloropropane; Docking; Molecular dynamics simulation