Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (4): 550-555.

• Articles • Previous Articles     Next Articles

Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

ZHENG Hai-tao1*, DONG Li-ping1, LIU Hai-hui1, JIA Hai-lian1 and SUYE Shin-ichiro2   

  1. 1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P. R. China;
    2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan
  • Received:2008-02-28 Revised:2008-06-16 Online:2009-07-25 Published:2009-10-16
  • Contact: ZHENG Hai-tao. E-mail: zhenght999@hotmail.com
  • Supported by:

    Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education, China and the Natural Science Foundation of Tianjin City, China(No.06YFJMJC15000).

Abstract:

Multilayer film was fabricated on an electrode surface by alternate layer-by-layer(LBL) adsorption of polycationic redox polymer(PEI-Fc) and dye-linked L-proline dehydrogenase(L-proDH). The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry, and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline. It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc. A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode, and the calculated currents fitted well with the experimental data. The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer. 

Key words: Electron communication; Dye-linked L-proline dehydrogenase; Layer-by-layer adsorption; Electrode kinetic