CRCU

Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (4): 483-486.

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Development and Characterization of Monoclonal Antibody Specifically Against TSP50

LIU Yang1,2, BAO Yong-li2, YU Chun-lei1, WU Yin1*, YANG Xiao-guang1, XU Hao-peng1,3, SUN Ying2 and LI Yu-xin2*   

  1. 1. National Engineering Laboratory for Druggable Gene and Protein Screening,
    2. Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education, Northeast Normal University, Changchun 130024, P. R. China;
    3. Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, New York, USA
  • Received:2009-01-16 Revised:2009-05-18 Online:2009-07-25 Published:2009-10-16
  • Contact: WU Yin. E-mail: wuy705@nenu.edu.cn; LI Yu-xin. E-mail: liyx486@nenu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30672068) and the Distinguished Young Scholars Fund of Jilin Province, China(No.20050114), the Key Grant of Jilin Province Science & Technology Committee, China(Nos. 20060923-01, 200805131) and the Key Grant of Changchun City Science & Technology Committee, China(No.06GG147).

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Cited

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Abstract:

Testis-specific protease 50(TSP50) has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples, which makes it an attractive molecular marker and a potential target for diagnosis and therapy. In the present study, we prepared a panel of monoclonal antibodies(mAbs) with high specificity and sensitivity against TSP50 by hybridoma method and characterized them by ELISA, Western blot, immunofluroescence and immunohistochemical analyses. The results show that all of the 9 different clones can specifically bind to TSP50. The mAbs against TSP50 we generated could be good tools for both basic and clinical studies.

Key words: Biomarker; Breast cancer; Monoclonal antibody; Testis-specific protease 50