CRCU

Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (4): 443-445.

• Articles • Previous Articles     Next Articles

Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor

WANG Xue-jiang1*, XIA Si-qing1, ZHAO Jian-fu1, ZHAO Hong-ning1 and RENAULT Nicole Jaffrezic2   

  1. 1. State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, P. R. China;
    2. Laboratoire de Sciences Analytiques(UMR CNRS 5180), Universite’Claude Bernard Lyon 1, Universite’de Lyon, Villeurbanne Cedex 69622, France
  • Received:2008-09-22 Revised:2008-10-14 Online:2009-07-25 Published:2009-10-16
  • Contact: WANG Xue-jiang. E-mail: wangxj@mail.tongji.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20707014) and the Opening Foundation of the Environmental Engineering Key Discipline, Zhejiang University of Technology, China(No.20080205).

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Abstract:

A conductometric nitrate biosensor based on methyl viologen/Nafion®/nitrate reductase(NR) interdigitated electrode for heavy metal determination was proposed. The activity of NR(EC 1.6.6.2) from Asper gillusniger(A. niger) was assayed as a function of metal concentration in the presence of Cu2+, Zn2+, Cd2+ and Pb2+. Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions. Detection limits for Cu2+, Zn2+, Cd2+ and Pb2+ were about 0.05, 0.5, 0.1 and 1.0 μmol/L, respectively. The results show that NR activity could not be regained after exposure to Cu2+, but could be partially recovered after exposure to Zn2+, Pb2+ and Cd2+.

Key words: Conductometric biosensor; Heavy metal; Nitrate reductase