Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (3): 347-352.

• Articles • Previous Articles     Next Articles

Adjuvant Effects of Dual Co-stimulatory Molecules on Cellular Responses to HIV Multiple-epitope DNA Vaccination

SHEN Zhen-wei1,2, JIN Hong-tao2, LI Chang2, CONG Yan-zhao2, NAN Wen-long2, BAI Liang2 and JIN Ning-yi2*   

  1. 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P. R. China;
    2. Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, P. R. China
  • Received:2008-04-28 Revised:2008-08-29 Online:2009-05-25 Published:2009-08-07
  • Contact: JIN Ning-yi, E-mail: ningyij@yahoo.com
  • Supported by:

    Supported by the National High-tech Research and Development Program(No.2006AA02Z447).

Abstract:

Designing adjuvants that can induce strong cytotoxic T cell responses is largely required for preparing DNA vaccines. In this study we explored dual costimulatory molecules 4-1BBL and OX40L as adjuvants to improve the efficiency of the HIV multiple-epitope DNA vaccine. When explored in the human dendritic cell-T cell based coculture system, dual costimulatory molecules significantly enhanced the anti-HIV T cell response of the HIV multiple-epitope DNA vaccine, as detected by intracellular cytokine staining to HIV antigens, cytokines accumulation in the cultures, and antigen-specific cytotoxic T lymphocyte responses. These results suggest that dual costimulatory molecules 4-1BBL and OX40L can effectively increase the potential of the HIV multiple-epitope antigen DNA vaccine and may provide an exciting approach for HIV therapy.

Key words: HIV DNA vaccine; Co-stimulatory molecules; 4-1BBL; OX40L; Dendritic cells