Chemical Research in Chinese Universities ›› 2009, Vol. 25 ›› Issue (3): 297-301.

• Articles • Previous Articles     Next Articles

Determination of Saponins in Leaf of Panax Ginseng C. A. Mey. by High Performance Liquid Chromatography

LIU Gui-ying1, ZHOU Hong-yu1, LU Juan1, ZHU Na1, GUI Ming-yu1, JIN Yong-ri1, ZHANG Yi-hua1, WANG Xin2 and LI Xu-wen1*   

  1. 1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China;
    2. Department of Radiation Medicine, School of Public Health, Jilin University, Changchun 130021, P. R. China
  • Received:2008-08-27 Revised:2008-10-08 Online:2009-05-25 Published:2009-08-07
  • Contact: LI Xu-wen, E-mail: xwli@jlu.edu.cn

Abstract:

A high performance liquid chromatography(HPLC) with UV detection was established for simultaneous determination of saponins in the leaf of Panax ginseng C. A. Mey. Nine ginsenosides(Rb1, Rb2, Rb3, Rc, Rd, F1, F2, F3, F5) and notoginsenoside Fe(NFe) were studied. Among the saponins, the ginsenosides F1, F2, F3, F5 and NFe were determined by HPLC-UV method for the first time. The determination of the ginsenosides via the HPLC-UV method was performed on a reversed-phase C18 column with gradient elution in 40 min. The linearity, precision, accuracy, and detection limit for determining the saponins were studied and the samples from different areas in China were analyzed. The HPLC-ESI-MS was used to identify the saponins. The results indicate that the HPLC-UV provided a good accuracy, reproducibility and sensitivity for the determination of the ten saponins.

Key words: Panax ginseng C. A. Mey.; Saponins; HPLC; Quantification; Determination