CRCU

Chemical Research in Chinese Universities ›› 2005, Vol. 21 ›› Issue (3): 298-300.

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Cloned s-Lap Gene Coding Area, Expression and Localizationof s-Lap/GFP Fusion Protein in Mammal Cells

SONG Yi-shu1, SONG Zhi-yu2, LI Hong-jun3, Wu Yin1, BAO Yong-li1, TAN Da-peng1, LI Yu-xin1   

  1. 1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, P. R. China;
    2. Department of Urology, the First Hospital, Jilin University, Changchun 130021, P. R. China;
    3. China-Japan Union Hospital, Jilin University, Changchun 130023, P. R. China
  • Received:2004-10-28 Online:2005-05-24 Published:2011-08-06
  • Supported by:

    Supported by the Postdoctor Science Foundation of China(No.2004035557)and the Natural Science Foundation of Jilin Province(No.20030541\|4).

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Abstract: s-Lap is a new gene sequence from pig retinal pigment epithelial(RPE) cells, which was found and cloned in the early period of apoptosis of RPE cells damaged with visible light. We cloned the coding area sequence of the novel gene of s-Lap and constructed its recombinant eukaryotic plasmid pcDNA3.1-GFP/s-lap with the recombinant DNA technique. The expression and localization of s-lap/GFP fusion protein in CHO and B16 cell lines were studied with the instantaneously transfected pcDNA3.1-GFP/s-lap recombinant plasmid. s-Lap/GFP fusion protein can be expressed in CHO and B16 cells with a high rate expression in the nuclei.

Key words: s-Lap gene, Fusion protein, Mammal cell, Expression, Localization