CRCU

Chemical Research in Chinese Universities ›› 2004, Vol. 20 ›› Issue (3): 314-316.

• Articles • Previous Articles     Next Articles

Analysis of Significance of Unite Examination of AFP and DNA Polymorphism of P3 Promoter of IGF-ⅡGene

LUO Su1,2, ZHANG Feng-chun1, SUN Chang-jiang3, LIU Cheng-bai2, ZHUANG Jiang-xing2,4, ZHANG Jin2   

  1. 1. Medical college, Beihua University, Jilin 132013, P. R. China;Collgege of Life Science, Jilin University, Changchun 130023, P. R. China;
    2. Medical college, Beihua University, Jilin 132013, P. R. China;
    3. Affiliated Hospital of Nantorg Medical College, Nantong Medical College, Nantong 226001, P. R. China;
    4. Zhangzhou Institute for Drug Control, Zhangzhou 363000, P. R. China
  • Received:2003-09-03 Online:2004-06-24 Published:2011-08-06
  • Supported by:

    Supported by the Department of Education of Jilin Province(No.200027).

PDF (PC)

Abstract: The DNA of P3 promoter region of IGF-Ⅱ gene was obtained by means of PCR technique. The examination of DNA polymorphism by restriction endonuclease BstE Ⅱ and the examination of AFP by bioluminescence immunoassay technique were carried out. The results have a significant difference(P<0.005). But the positive rate of AFP is higher than that of DNA polymorphism. The experimental result shows that the change of the DNA polymorphism of IGF-Ⅱis not the only carcinogenic factor. The suggested unite examination is the best method for the diagnosis of the primary hepatocellular carcinoma.

Key words: Primary hepatocellular carcinoma, α-Fetalglobulin AFP, Insulin-like growth factorⅡ(IGF-Ⅱ), DNA polymorphism, Bioluminescence immunoassay technique