CRCU

Chemical Research in Chinese Universities ›› 2003, Vol. 19 ›› Issue (3): 317-319.

• Articles • Previous Articles     Next Articles

Chemical Modification of Amino Acid Residues in Human Plasminogen

LIANG Fang1, SUN Hong2, ZHAO Cheng-guang1, CUI Ting1, HONG Shui-sheng1, CHEN Jia1, LIU Lan-ying1   

  1. 1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
    2. Changchun Institute of Biological Products, Changchun 130062, P. R. China
  • Received:2002-09-05 Online:2003-08-24 Published:2011-08-06
  • Supported by:

    Supported by the Natural Science Foundation of Jilin Province(No.030912).

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Abstract: The chemical modification of human plasminogen(HPg) was studied with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide(EDC), N-acetylimidazole(NAI), 1,2-cyclohexanedione(CHD), chloramine T(Ch-T) and N-bromosuccinimide(NBS) as modifying reagents at its carboxyl group, tyrosine, arginine, methionine and tryptophan residues, respectively. The results indicate that tyrosine and arginine residues are not essential for HPg activity, while carboxyl groups, methionine and tryptophan residues are important for the activity of HPg. The Keech and Farrant′s kinetic analysis reveals that one tryptophan residue, one methionine residue and two carboxyl groups are essential for HPg activity.

Key words: Human plasminogen(HPg), Chemical modification, Carboxyl group, Methionine, Tryptophan