CRCU

Chemical Research in Chinese Universities ›› 1999, Vol. 15 ›› Issue (1): 39-46.

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Synthesis of Novel Hydrophobic Media and Purification of Recombinant HBsAg

WANG Yan1, WANG Qun1, XIAO Neng-qing2, LIU Da-wei1, YE Shi-de1, LUO Xuan1, GUAN Gui-fan1, WEI Zi-li1, CHEN Wan-ge1, BAI Fang3   

  1. 1. Changchun Institute of Biological Products, Changchun, 130062;
    2. Department of Biochemistry, Peking University, Beijing, 100871;
    3. Shenyang Rust Proof Packaging Material Company, Shenyang, 110032
  • Received:1997-09-19 Online:1999-01-24 Published:2011-08-17

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Abstract: A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r-HBsAg). r-HBsAg was further purified to the final products by following a downstream procedure[1]. The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r-HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r-HBsAg products have reached the same level as other similar kinds of products.

Key words: Chinese hamster ovary(CHO), recombinant hepatitis B surface antigen(r-HBsAg), hydrophobic interaction chromatography(HIC), reverse phase haemagglutination assay(RPHA)