Synthesis and Characterization of Specific Haptens Used to Generate Abzyme with Glutathione Peroxidase Activity

Chemical Research in Chinese Universities ›› 1998, Vol. 14 ›› Issue (3): 277-279.

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Synthesis and Characterization of Specific Haptens Used to Generate Abzyme with Glutathione Peroxidase Activity

Liu Jun-qiu, Gao Shu-juan, Luo Gui-min, Shen Jia-cong 

The National Laboratory of Enzyme Engineering, Key Laboratory for Supermolecular Spectra, Structure of Jilin University, Jinlin University, Changchun 130023

Received:1997-06-27 Online:1998-08-24 Published:2011-08-17
Supported by:
Supportedby 863 Foundation.

Abstract

Abstract: Glutathione was modified selectively by 2,4-dinitrochlorobenzene, giving S-substituted dinitrophenyl glutathione(GSH-S-DNP). GSH-S-DNP was further esterified by iso-butanol, hexanol, cyclohexanol and benzyl alcohol. Four haptens used to generate abzyme with glutathione peroxidase(GPX) activity were synthesized. They are GSH-DNP biesters: GSH-DNP-diiso-butyl ester(GSH-DNP-IBU), GSH-DNP bihexyl ester(GSH-DNP- HE), GSH-DNP bicyclohexyl ester(GSH-DNP-CH), GSH-DNP-bibenzylmethyl ester(GSH-DNP-BE). The structures of the haptens were characterized by means of elemental analysis, IR and ¹H NMR.

Key words: Glutathione peroxidase, Abzyme, Hapten

Liu Jun-qiu, Gao Shu-juan, Luo Gui-min, Shen Jia-cong  . Synthesis and Characterization of Specific Haptens Used to Generate Abzyme with Glutathione Peroxidase Activity[J]. Chemical Research in Chinese Universities, 1998, 14(3): 277-279.

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Synthesis and Characterization of Specific Haptens Used to Generate Abzyme with Glutathione Peroxidase Activity

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