Abstract: The chemiluminescence reaction between potassium permanganate and brucine was thoroughly investigated, and a new method for brucine determination based on the chemiluminescence reaction was established. The method possesses the poten- tial advantages of simplicity on instrument, high sensitivity and precision, commer-ically available reagents to be used, and is suitable for on-line analysis. The linear range and detection limit of this method are from 4 ×10^-9 to 8×10^-5 g·mL^-1 and 1. 5 ×10^-9 g·mL^-1, respectively. The standard deviation in the whole linear range is less than 2.0%. This method has been used to determine the brucine in nux vom- ica with a recovery ratio of 97% to 108%.

Key words: Chemiluminescence, Brucine, Toxicological analysis