Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

doi:10.1007/s40242-023-3046-9

Chemical Research in Chinese Universities ›› 2023, Vol. 39 ›› Issue (3): 472-480.doi: 10.1007/s40242-023-3046-9

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Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

WANG Hao, ZHANG Yue, ZHAO Liuqing, HE Zeying, ZHANG Jingran, LIU Bingjie, LIU Xiaowei, ZHANG Yanwei

1. Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, P. R. China;
2. Shanghai SCIEX Analytical Instrument Trading Co., Ltd., Shanghai, 200335, P. R. China;
3. Department of Applied Chemistry, China Agricultural University, Beijing, 100193, P. R. China

Received:2023-02-27 Published:2023-05-25
Contact: Yue Zhang, Yanwei Zhang E-mail:17854166935@163.com;zhangyanwei@caas.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No.41807391) and the Tianjin Natural Science Foundation, China (No.18JCQNJC09100).

Abstract

Abstract: The ecotoxicity of perfluorooctane sulfonate(PFOS) to plants has been reported, but the lack of study on the effect of PFOS isomers is of concern. In this paper, the effect mechanism of Arabidopsis thaliana(A. thaliana) against two different concentrations of L-PFOS or perfluoro-3-methylheptanesulfonate (P3MHpS) was investigated via metabolomics. The metabolism of Arabidopsis thaliana leaves was investigated via the metabolomics strategy and statistical analysis. And 41 biomarkers for L-PFOS and 32 biomarkers for P3MHpS were found and identified. There was no significant difference of saccharides between L-PFOS and P3MHpS. More amino acids were affected by P3MHpS than by L-PFOS in high-level exposure group, while the opposite phenomenon was observed in low-level exposure group. More phytohormones were affected by L-PFOS than by P3MHpS in the high-level exposure group, however, the elevated level of indole phytohormones was significantly higher by P3MHpS than by L-PFOS. The level of up-regulation of (poly)phenols in L-PFOS group was significantly higher than that in P3MHpS group. The affected aliphatic glucosinolate metabolism was firstly observed with higher up-regulation induced by L-PFOS than by P3MHpS. New insight of phytotoxicity of PFOS isomers to plants was provided.

Key words: L-Perfluorooctane sulfonate(L-PFOS), Perfluoro-3-methylheptanesulfonate(P3MHpS), Phytotoxicity, Metabolomics, Glucosinolate

WANG Hao, ZHANG Yue, ZHAO Liuqing, HE Zeying, ZHANG Jingran, LIU Bingjie, LIU Xiaowei, ZHANG Yanwei. Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics[J]. Chemical Research in Chinese Universities, 2023, 39(3): 472-480.

References

[1] Chou W.-C., Lin Z., Environment International, 2020, 137, 105581
[2] Lindim C., van Gils J., Cousins I. T., Water Research, 2016, 103, 124
[3] Rovira J., Angeles Martinez M., Prasad Sharma R., Espuis T., Nadal M., Kumar V., Costopoulou D., Vassiliadou I., Leondiadis L., Domingo J. L., Schuhmacher M., Environmental Research, 2019, 175, 384
[4] Johansson N., Fredriksson A., Eriksson P., Neurotoxicology, 2008, 29, 160
[5] Xu D., Li C., Wen Y., Liu W., Environmental Pollution, 2013, 174, 121
[6] Li P., Oyang X., Xie X., Li Z., Yang H., Xi J., Guo Y., Tian X., Liu B., Li J., Xiao Z., Journal of Hazardous Materials, 2020, 389, 121852
[7] Guo Q., He Z., Liu X., Liu B., Zhang Y., Science of the Total Environment, 2020, 710, 135542
[8] Greaves A. K., Letcher R. J., Chemosphere, 2013, 93, 574
[9] Sharpe R. L., Benskin J. P., Laarman A. H., MacLeod S. L., Martin J. W., Wong C. S., Goss G. G., Environmental Toxicology and Chemistry, 2010, 29, 1957
[10] Zhong W., Zhang L., Cui Y., Chen M., Zhu L., Science of the Total Environment, 2019, 647, 992
[11] Chu S., Letcher R. J., Analytical Chemistry, 2009, 81, 4256
[12] Zheng P., Liu M., Yin H., Shen L., Wang Y., Wu Q., Environmental Pollution, 2020, 267, 115381
[13] Zhang Y., Tan D., Geng Y., Wang L., Peng Y., He Z., Xu Y., Liu X., International Journal of Environmental Research and Public Health, 2016, 13, 1224
[14] Schulz K., Silva M. R., Klaper R., Science of the Total Environment, 2020, 733, 139186
[15] Li P., Li J., Science of the Total Environment, 2021, 770, 144726
[16] Du W., Liu X., Zhao L., Xu Y., Yin Y., Wu J., Ji R., Sun Y., Guo H., Science of the Total Environment, 2020, 724, 138257
[17] Li P., Xiao Z., Sun J., Oyang X., Xie X., Li Z., Tian X., Li J., Science of the Total Environment, 2020, 726, 138382
[18] Kruger N. J., von Schaewen A., Current Opinion in Plant Biology, 2003, 6, 236
[19] Carpenter J. F., Crowe J. H., Arakawa T., Journal of Dairy Science, 1990, 73, 3627
[20] D’Andrea G., Fitoterapia, 2015, 106, 256
[21] Likic S., Sola I., Ludwig-Mueller J., Rusak G., European Journal of Plant Pathology, 2014, 138, 257
[22] Li H., Yao Y., Li L., Journal of Pharmacy and Pharmacology, 2017, 69, 1253
[23] Singh A., Singh I. K., Molecular Aspects of Plant-pathogen Interaction(Vol. 10), Springer, New Delhi, 2018
[24] O’Brien J. A., Benkova E., Frontiers in Plant Science, 2013, 4, 451
[25] Khan M. I. R., Fatma M., Per T. S., Anjum N. A., Khan N. A., Frontiers in Plant Science, 2015, 6, 462
[26] Halkier B. A., Gershenzon J., Annual Review of Plant Biology, 2006, 57, 303
[27] Bones A. M., Rossiter J. T., Phytochemistry, 2006, 67, 1053
[28] Beesoon S., Martin J. W., Environmental Science & Technology, 2015, 49, 5722

Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

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