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Chemical Research in Chinese Universities ›› 2023, Vol. 39 ›› Issue (3): 441-448.doi: 10.1007/s40242-023-3032-2

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RNA-sequencing-based Gene Expression Profile Revealing Breast Tumor Development Induced by Exposure of Bisphenol S

ZHOU Peng, XIAO Yu, ZHOU Xin, LIU Jianjun, ZHAO Chao   

  1. 1. Bionic Sensing and Intelligence Center, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China;
    2. Department of Ultrasound, The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen Second People's Hospital, Shenzhen 518035, P. R. China;
    3. Department of Breast and Thyroid Surgery, Shenzhen Second People's Hospital/The First Hospital Affiliated to Shenzhen University, Shenzhen 518035, P. R. China;
    4. Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of Health Toxicology(2020-2024), Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, P. R. China;
    5. University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
    6. Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China;
    7. Key Laboratory of Molecular Epidemiology of Hunan Province, School of Medicine, Hunan Normal University, Changsha 410013, P. R. China
  • Received:2023-02-17 Online:2023-06-01 Published:2023-05-25
  • Contact: ZHAO Chao E-mail:chao.zhao@siat.ac.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (No.22176195), the Natural Science Foundation of Guangdong Province, China (No.2021A1515010171), the Key Program of Fundamental Research in Shenzhen, China(No.JCYJ20210324115811031), the Project of the Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression, China (No.ZDSYS20220606100606014), the Shenzhen Key Medical Discipline Construction Fund, China(No.SZXK052), the Clinical Research Project of Shenzhen Second People’s Hospital, China(No.20203357001), and the Guangdong Basic and Applied Basic Research Foundation, China (No.2021A1515110096).

Abstract: Aberrant biological information occurs naturally at exposure to bisphenol A or its alternatives, which was associated with the occurrence and development of breast cancer. However, the potential molecular variation in gene expression during the breast tumor development is still unclear. Herein, high throughput RNA sequencing(RNA-seq) and bioinformatics analysis were used to investigate the variation of tumor-mRNA profile exposed with BPS5 (5 μg/kg bw/day) or BPS50(50 μg/kg bw/day) in tumor development-associated MMTV-PyMT transgenic mouse model. Meanwhile, we analyzed the dose-effects of bisphenol S(BPS) and BPS-induced tumor development on the gene level exhaustively. In dose-effect aspects of BPS, the increased concentration of BPS significantly changed the numbers and enrichment pathway of differentially expressed genes(DEGs), especially the enrichment pathways involved in up-regulated genes including ribosome, peroxisome proliferators-activated receptor(PPAR) signaling pathway and progesterone-mediated oocyte maturation pathway. In effects of BPS exposure to tumor development, expression of IgκC, Zfp385b, Cldn10, Pgr and Snord14d has changed significantly throughout the tumor development. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) results obtained from BPS-induced tumor development showed that the functional classifications were intensively altered with an extension of time in high-dose BPS groups. The acquired DEGs and pathway information could help with the accurate exploration of molecular mechanisms of tumor development, screening of molecular targets of breast cancer, and toxicological evaluation of environmental pollutants.

Key words: Bisphenol S, RNA-sequencing, Breast cancer, Tumor development