Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC

doi:10.1007/s40242-019-8284-5

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (1): 53-59.doi: 10.1007/s40242-019-8284-5

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Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC

SONG Yunxi¹, SONG Zhen², YANG Binsheng¹

1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, P. R. China;
2. Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, P. R. China

Received:2018-09-10 Revised:2018-10-07 Online:2019-02-01 Published:2018-11-26
Contact: YANG Binsheng E-mail:yangbs@sxu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (Nos.21571117, 21701121).

Abstract

Abstract: Interactions among Cu (Ⅱ), doxorubicin and copper operon C (CopC) have been investigated in detail by means of fluorescence, UV-Vis, IR spectra, isothermal titration calorimetry (ITC) and molecular docking in Tris-HCl buffer (50 mmol/L, pH=7.4, 25℃). The results suggest that Cu (Ⅱ)-doxorubicin is formed in a Cu (Ⅱ) to doxorubicin molar ratio of 1:2, and the conditional stability constant, K_{[Cu (Ⅱ)-doxorubicin]} is 1.90×10⁹ L²/mol², CopC and doxorubicin can form a 1:1 complex, the conditional stability constant is greater than 10⁵ L/mol. Binding of doxorubicin causes a conformational change in CopC with the reduction of β-sheet and increase of random coil, and the stability of CopC is decreased. Cu (Ⅱ), doxorubicin and CopC can form a CopC-Cu (Ⅱ)-doxorubicin ternary complex. The formation of CopC-Cu (Ⅱ)-doxorubicin reduced greatly the reduction rate of Cu (Ⅱ) by ascorbate (Vc), i.e., the binding of doxorubicin affects the action of CopC as redox switch.

Key words: Doxorubicin, Copper operon C (CopC), Cu (II) interaction

SONG Yunxi, SONG Zhen, YANG Binsheng. Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC[J]. Chemical Research in Chinese Universities, 2019, 35(1): 53-59.

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Spectral Study on the Interactions Among Cu(II), Doxorubicin and CopC

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